DNA Polymerase
1000U/200ml 5U/ml

DNA ploymerase is a thermo stable enzyme of isolated from Thermos aquatics. This enzyme contain 5`-3` polymerase and 5`-3` exonuclease activity.
Storage buffer: 50mM Tris-HCl pH7.9, 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mM PMSF, 50% glycerol.
10X reaction buffer:
buffer A: containing 15mM MgCl₂
buffer B: without MgCl₂
Storage Condition:-20℃
Unit description:
One unit is defined as the amount of enzyme that will incorporate 10n mole of dNTP into acid-insoluble material in 30 minutes at 74℃.
The reaction conditions are:
50mM Tris-HCl pH8.8, 50mM NaCl, 5mM MgCl₂, 200uM each of dATP, dCTP, H³dTTP, 10ug activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50ul.
Source: Thermus aquaticus YTl